Download >> Download Targetron gene knockout system user guide
Read Online >> Read Online Targetron gene knockout system user guide
The TargeTron Gene Knockout System is a revolutionary method for rapid and specific disruption of genes in prokaryotic organisms. Utility of the technology has been demonstrated for prokaryotic genetic engineering, systems biology and functional genomics approaches.
To better understand and improve the biochemical pathway to solvents, the development of genetic tools for engineering C. beijerinckii is highly desired. Based on mobile group II intron technology, a targetron gene knockout system was developed for C. beijerinckii in this study. This system was successfully employed to
I'm using pET-TORG/RIG plasmid (Coros, Colin J., et al. "Global regulators orchestrate group II intron retromobility." Molecular cell 34.2 (2009): 250-256.) on both C41 DE3 (derivative of BL21 DE3) and K12 MG1655 strains. The plasmid has Amp resistance for pos. selection of transformation and the Intron contains Kan
12 Nov 2015
mutated to re-target insertion into a user-specified chromosomal gene. A somewhat accurate analogy is that group II introns are like programmable restriction enzymes, with the added activity of inserting RNA into a cleaved DNA sequence. Figure 1 outlines the process of using TargeTron system to knockout bacterial genes.
19 Jul 2013 To better understand and improve the biochemical pathway to solvents, the development of genetic tools for engineering C. beijerinckii is highly desired. Based on the mobile group II intron technology, a Targetron gene knockout system was developed for C. beijerinckii in this study. This system was
19 Jul 2013 Based on the mobile group II intron technology, a Targetron. 31 gene knockout system was developed for C. beijerinckii in this study. This system was. 32 successfully employed to disrupt .. DNA Labeling and Detection Starter Kit, according to the instruction manual (Roche. 173. Diagnostics, Mannheim
The intron on pACD4K-C was re-targeted to lacZ using the control primers provided in the Targetron Gene Knockout System kit (Sigma) according to the manufacturer's instructions, yielding the plasmid pACD4K-ClacZTT. The thl promoter of C. acetobutylicum ATCC 824 was PCR-amplified from pSOS95 (Tummala et al.,
TargeTron™ Gene Knockout System. 3 ea. 10 ea. Note: Reagents in the 3 ea kit are sufficient for 3 designs, 12 reactions. Reagents in the 10 ea kit are sufficient for 10 designs, 40 reactions. Visit our web site at sigma-aldrich.com/targetron for updates on new TargeTron products and protocols. Future releases may include.
For the spc promoter, modify the existing protocol (included in the TargeTron Gene Knockout System User Guide) by taking the transformation from the 1 hr 37 °C incubation, diluting 100 µl into 3 ml of LB-chloramphenicol-glucose, and grow overnight at 30 °C. After overnight growth, spin down the cells and resuspend in
https://www.flickr.com/groups/3783156@N20/discuss/72157666772408069/ https://www.flickr.com/groups/3760701@N25/discuss/72157690098761005/ http://forum.us.kick9.com/viewtopic.php?f=46&t=795342 http://xsjptil.mmohost.ru/viewtopic.php?id=104 http://fanclub.quiho.com/m/events/view/Cetina-canyon-hiking-guide